Specific down-regulation of PAL genes by artificial microRNAs in Populus trichocarpa.
Identifieur interne : 003114 ( Main/Exploration ); précédent : 003113; suivant : 003115Specific down-regulation of PAL genes by artificial microRNAs in Populus trichocarpa.
Auteurs : Rui Shi [États-Unis] ; Chenmin Yang ; Shanfa Lu ; Ronald Sederoff ; Vincent L. ChiangSource :
- Planta [ 1432-2048 ] ; 2010.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Populus.
- physiologie : microARN.
- Amorces ADN, Gènes de plante, Mutation, RT-PCR, Régulation négative, Similitude de séquences d'acides nucléiques, Séquence nucléotidique.
English descriptors
- KwdEn :
- MESH :
- chemical , physiology : MicroRNAs.
- chemical : DNA Primers.
- genetics : Populus.
- Base Sequence, Down-Regulation, Genes, Plant, Mutation, Reverse Transcriptase Polymerase Chain Reaction, Sequence Homology, Nucleic Acid.
Abstract
Artificial microRNAs (amiRNAs) are similar to microRNAs (miRNAs) in that they are able to reduce the abundance of specific transcripts in plants by RNA-Induced Silencing Complex (RISC)-mediated cleavage and degradation, but differ in that they are designed for specific targets. The long generation times of forest trees have limited the discovery of mutations by conventional genetics. AmiRNAs can create gene-specific transcript reduction in transgenic trees in a single generation and may have broad application for functional genomics of trees. In this paper, we describe the specific down-regulation of multiple genes in the phenylalanine ammonia-lyase (PAL) gene family of Populus trichocarpa using amiRNA sequences incorporated in a P. trichocarpa miRNA-producing precursor, ptc-MIR408. Two different amiRNA constructs were designed to specifically down-regulate two different subsets of PAL genes, revealing differential regulation within the gene family. Down-regulation of subset A (PAL2, PAL4 and PAL5) by amiRNA-palA led to an increase in transcript abundance of subset B (PAL1 and PAL3). The reciprocal effect was not observed.
DOI: 10.1007/s00425-010-1253-3
PubMed: 20725738
Affiliations:
Links toward previous steps (curation, corpus...)
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The long generation times of forest trees have limited the discovery of mutations by conventional genetics. AmiRNAs can create gene-specific transcript reduction in transgenic trees in a single generation and may have broad application for functional genomics of trees. In this paper, we describe the specific down-regulation of multiple genes in the phenylalanine ammonia-lyase (PAL) gene family of Populus trichocarpa using amiRNA sequences incorporated in a P. trichocarpa miRNA-producing precursor, ptc-MIR408. Two different amiRNA constructs were designed to specifically down-regulate two different subsets of PAL genes, revealing differential regulation within the gene family. Down-regulation of subset A (PAL2, PAL4 and PAL5) by amiRNA-palA led to an increase in transcript abundance of subset B (PAL1 and PAL3). The reciprocal effect was not observed.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20725738</PMID><DateCompleted><Year>2011</Year><Month>03</Month><Day>28</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1432-2048</ISSN><JournalIssue CitedMedium="Internet"><Volume>232</Volume><Issue>6</Issue><PubDate><Year>2010</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Planta</Title><ISOAbbreviation>Planta</ISOAbbreviation></Journal><ArticleTitle>Specific down-regulation of PAL genes by artificial microRNAs in Populus trichocarpa.</ArticleTitle><Pagination><MedlinePgn>1281-8</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00425-010-1253-3</ELocationID><Abstract><AbstractText>Artificial microRNAs (amiRNAs) are similar to microRNAs (miRNAs) in that they are able to reduce the abundance of specific transcripts in plants by RNA-Induced Silencing Complex (RISC)-mediated cleavage and degradation, but differ in that they are designed for specific targets. The long generation times of forest trees have limited the discovery of mutations by conventional genetics. AmiRNAs can create gene-specific transcript reduction in transgenic trees in a single generation and may have broad application for functional genomics of trees. In this paper, we describe the specific down-regulation of multiple genes in the phenylalanine ammonia-lyase (PAL) gene family of Populus trichocarpa using amiRNA sequences incorporated in a P. trichocarpa miRNA-producing precursor, ptc-MIR408. Two different amiRNA constructs were designed to specifically down-regulate two different subsets of PAL genes, revealing differential regulation within the gene family. Down-regulation of subset A (PAL2, PAL4 and PAL5) by amiRNA-palA led to an increase in transcript abundance of subset B (PAL1 and PAL3). 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Chiang</name><name sortKey="Lu, Shanfa" sort="Lu, Shanfa" uniqKey="Lu S" first="Shanfa" last="Lu">Shanfa Lu</name><name sortKey="Sederoff, Ronald" sort="Sederoff, Ronald" uniqKey="Sederoff R" first="Ronald" last="Sederoff">Ronald Sederoff</name><name sortKey="Yang, Chenmin" sort="Yang, Chenmin" uniqKey="Yang C" first="Chenmin" last="Yang">Chenmin Yang</name></noCountry><country name="États-Unis"><region name="Caroline du Nord"><name sortKey="Shi, Rui" sort="Shi, Rui" uniqKey="Shi R" first="Rui" last="Shi">Rui Shi</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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